Hematopoietic cells emerging from hemogenic endothelium exhibit lineage-specific oxidative stress responses

During human embryogenesis, distinct waves of hematopoiesis give rise to various blood cell types, originating from hemogenic endothelial (HE) cells. As HE cells reside in hypoxic conditions in the embryo, we investigated the role of hypoxia in human EHT and subsequent hematopoiesis. Using single-cell RNA sequencing we describe hypoxia-related transcriptional changes in different HE-derived blood lineages, which reveal that erythroid cells are particularly susceptible to oxidative stress, due to decreased NRF2 activity in hypoxia. In contrast, non-erythroid CD45