Abstract In this work, Zn‐incorporated titanium substrate with nano network structures was built, and its antibacterial activity against the gram positive and negative bacteria was tested. Ti plate was successively treated by hot alkaline, Zn 2+ ion‐exchange, and ZnO nanoparticles deposit, where alkaline treated Ti plate (NTi), Zn 2+ exchanged Ti plate (NZTi), Zn 2+ /ZnO incorporated Ti plate (NZZTi) were correspondingly generated. For Escherichia coli , the antibacterial percentage of NZTi and NZZTi after 24 h was 99.86 % and 99.98 %, while it was 99.95 % and 100 % for Staphylococcus aureus , respectively. The result disclosed that NZZTi have a bit better antibacterial activity than NZTi, which indicates that Zn 2+ shows fabulous antibacteria activity and the presence of ZnOnps can contribute the antibacterial ability of Ti plates, which agrees with result of NZZTi‐Cup antibacterial effect. The release tests of Zn 2+ after 8 days from NZZTi‐Cup disclosed that Zn‐incorporated Ti plate could consecutively release trace Zn ions.