拟杆菌
生物
专性厌氧菌
鼠李糖乳杆菌
罗伊乳杆菌
拟杆菌
微生物群
计算生物学
微生物学
人类健康
人体微生物群
串扰
人体胃肠道
使负有责任或义务
代谢组
益生菌
细胞生物学
生物信息学
细菌
遗传学
代谢组学
医学
光学
物理
环境卫生
生态学
作者
Pranjul Jaykumar Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh S. Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew D. Estes,Christian Jäger,Carole Seguin-Devaux,Frédéric Zenhausern,Paul Wilmes
摘要
Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human-microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human-microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host-microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.
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