Identification of differentially expressed microRNAs in peripheral blood mononuclear cells of systemic lupus erythematosus patients

Objective To investigate the differentially expressed microRNAs(miRNA) in peripheral mononuclear cells(PBMCs) in the systemic lupus erythematosus(SLE) patients.Methods PBMCs were isolated from blood samples of 23 SLE patients and 10 health controls.The miRNA extracted from PBMC was applied to the miRNA microarray chip.Four random differentially expressed miRNAs were verified by Northern blot analysis.Results There were 14 miRNAs differentially expressed were indentified in SLE according to microarray chip.These results were confirmed by Northern blot analysis.Conclusion The altered expression profile of miRNA in PBMCs of SLE patients implies differentially expressed miRNA plays roles in the pathogenesis of SLE.