HPLC determination of s-isomer in levocetirizine hydrochloride and its tablets

Objective:To determine s-isomer in levocetirizine hydrochloride and its tablets by HPLC.Method:A Chiral-AGP column was used.The mobile phase was composed of 0.01 mol·L~(-1)buffer of potassium phosphate (pH 7.1)-acetonitrile(93:7)with flow rate of 0.5 mL·min~(-1)at detection wavelength of 230 nm.Result:Levo- cetirizine hydrochloride and its s-isomer was separated completely,the detection limit was 0.9 ng and the recovery of s-isomer was 98.0%.Conclusion:This method is rapid,simple,and can be used for determination s-isomer in levocetirizine hydrochloride and its tablets.