检出限
表面增强拉曼光谱
糖蛋白
基质(水族馆)
化学
拉曼光谱
分子
材料科学
纳米技术
核化学
生物物理学
分析化学(期刊)
色谱法
拉曼散射
生物化学
光学
物理
生物
有机化学
生态学
作者
Ting Zhou,Dechan Lu,Qiutian She,Cairou Chen,Jingbo Chen,Zufang Huang,Shangyuan Feng,Ruiyun You,Yudong Lu
标识
DOI:10.1016/j.snb.2021.129597
摘要
We successfully prepared a two-dimensional surface enhanced Raman spectroscopy(SERS) substrate for the trace detection of Interleukin-6(IL-6) in plasma. Glycoproteins in serum were captured by a two-dimensional silver plate modified with 4-mercaptobenzoic acid (4-MPBA). And then, the [email protected] core-shell nanoprobes modified with IL-6 antibody accurately recognized IL-6 glycoprotein. The [email protected] NPs were embedded with Prussian Blue (PB) molecule, which only peaked at 2172 cm−1 in the biological silence zone. The calibration model of 4-MPBA and PB double signal molecules can avoid the interference of other glycoprotein macromolecules in the environment, thus realizing the quantitative detection of IL-6. The results showed that the higher the IL-6 content, the stronger the Raman signal. And the detection limit of IL-6 in serum can reach 5 pg/mL, the applicable range is 0.5–50000 pg/mL (R2 = 0.9928). Therefore, this method can be used as a multifunctional SERS activity technology, which can be used to detect IL-6 ultra-sensitively and quantitatively.
科研通智能强力驱动
Strongly Powered by AbleSci AI