Two-Cistronic Expression Plasmids for High-Level Gene Expression in Escherichia coli Preventing Translational Initiation Inhibition Caused by the Intramolecular Local Secondary Structure of mRNA

Journal Article Two-Cistronic Expression Plasmids for High-Level Gene Expression in Escherichia coli Preventing Translational Initiation Inhibition Caused by the Intramolecular Local Secondary Structure of mRNA Get access Shigenobu Kimura, Shigenobu Kimura Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Hyogo 678-1297 *To whom correspondence should be addressed at the present address: Department of Biomolecular Functional Engineering, Faculty of Engineering, Ibaraki University, 4-12-1 Nakanarusawa-cho, Hitachi, Ibaraki 316-8511. Tel: +81-294-38-5062, Fax: +81-294-38-5072, E-mail: s-kimura@mx.ibaraki.ac.jp Search for other works by this author on: Oxford Academic PubMed Google Scholar Tomoka Umemura, Tomoka Umemura Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Hyogo 678-1297 Search for other works by this author on: Oxford Academic PubMed Google Scholar Takashi Iyanagi Takashi Iyanagi Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Hyogo 678-1297 Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Biochemistry, Volume 137, Issue 4, April 2005, Pages 523–533, https://doi.org/10.1093/jb/mvi065 Published: 01 April 2005 Article history Received: 16 November 2004 Accepted: 11 February 2005 Published: 01 April 2005