生物传感器
肠毒素
表面等离子共振
检出限
缓冲器(光纤)
色谱法
化学
微生物学
材料科学
纳米技术
生物
纳米颗粒
大肠杆菌
生物化学
基因
电信
计算机科学
作者
Jiřı́ Homola,Jakub Dostálek,Shengfu Chen,Avraham Rasooly,Shaoyi Jiang,Sinclair S. Yee
标识
DOI:10.1016/s0168-1605(02)00010-7
摘要
This work evaluates a newly developed wavelength modulation-based SPR biosensor for the detection of staphylococcal enterotoxin B (SEB) in milk. Two modes of operation of the SPR biosensor are described: direct detection of SEB and sandwich assay. In the sandwich assay detection mode, secondary antibodies are bound to the already captured toxin to amplify sensor response. Samples including SEB in buffer and SEB in milk were analyzed in this work. The SPR biosensor has been shown to be capable of directly detecting concentrations of SEB in buffer as low as 5 ng/ml. In sandwich detection mode, the lowest detection limit was determined to be 0.5 ng/ml for both buffer and milk samples. The reported wavelength modulation-based SPR sensor provides a generic platform which can be tailored for detection of various foodborne pathogens and agents for food analysis and testing.
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