Effect of Irradiated Sucrose on Carbohydrate Metabolism of Carrot Cells

FAUST, Mi., CHASE, B. R., AND MASSEY, L. Mi., JR. Effect of Irradiated Sucrose on Carbohydrate Metabolism of Carrot Cells. Radiation Res. 31, 201-205 (1967). The possible indirect effect of radiation through radiolysis of sucrose on carbohydrate metabolism of carrot cells was investigated. This was accomplished by study of the utilization of specifically labeled substrates in the presence of y-irradiated aqueous sucrose. It was found that the presence of irradiated sucrose did not cause abnormality in the utilization of labeled glucose, acetate, or succinate. An apparent effect of irradiated sucrose on utilization of labeled glucose proved to be an artifact, probably due to radiolytic formation of glucose from sucrose. It was concluded that the Embden-Meyerhof-Paranas pathway, the pentose phosphate pathway, and the citric acid cycle of carrot cells were not affected by the presence of irradiated sucrose.