Studies on the retention behavior of quercetin, phenol, and caffeine as test substances on selected neutral and charged hydrophilic interaction liquid chromatography stationary phases

化学 范迪姆特方程 色谱法 相(物质) 苯酚 乙腈 吸附 分析化学(期刊) 甲醇 柱色谱法 高效液相色谱法 有机化学
作者
P. Ziobrowski,Lidia Zapała,Wojciech Zapała
出处
期刊:Separation science plus [Wiley]
卷期号:5 (7): 267-274 被引量:2
标识
DOI:10.1002/sscp.202200027
摘要

Abstract The influence of mobile phase composition and column temperature on the retention behavior of quercetin, phenol, and caffeine as test analytes was analyzed. The experiments were conducted in columns filled with the charged amine phase and the neutral amide phase. As mobile phases, the varying fractions of methanol or acetonitrile in water solutions were applied. The most likely retention mechanisms have been discussed. The results of investigations confirm the importance of a mixed adsorption‐partition retention mechanism in the hydrophilic interaction liquid chromatography process, regardless of the mobile phase composition and type of stationary phase. Investigations have also shown that in the tested systems the retention mechanism is also strongly column temperature‐dependent. Additionally, the efficiency of the tested columns was determined and analyzed as a function of the linear flow velocity. The obtained literature known as van Deemter plots confirms generally higher column efficiencies in the systems with acetonitrile as an organic component of the mobile phase. However, in some cases, the application of methanol allows obtaining similar column efficiencies. Furthermore, the results show that both columns tested exhibit comparable efficiency in the most tested systems and provide very good efficiency preservation over the wide range of eluent velocities.

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