肠道菌群
动态成像
体内
生物
化学
生物化学
图像处理
计算机科学
数字图像处理
图像(数学)
生物技术
人工智能
作者
Dongxia Chen,Junnan Guo,Ao Li,Chengjie Sun,Huibin Lin,Hongyu Lin,Chaoyong Yang,Wei Wang,Jinhao Gao
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2023-01-27
卷期号:9 (4)
被引量:10
标识
DOI:10.1126/sciadv.abg6808
摘要
Real-time localization and microbial activity information of indigenous gut microbiota over an extended period of time remains a challenge with existing visualizing methods. Here, we report a metabolic fluorine labeling (MEFLA)-based strategy for monitoring the dynamic gut microbiota via 19F magnetic resonance imaging (19F MRI). In situ labeling of different microbiota subgroups is achieved by using a panel of peptidoglycan-targeting MEFLA probes containing 19F atoms of different chemical shifts, and subsequent real-time in vivo imaging is accomplished by multiplexed hotspot 19F MRI with high sensitivity and unlimited penetration. Using this method, we realize extended visualization (>24 hours) of native gut microbes located at different intestinal sections and semiquantitative analysis of their metabolic dynamics modulated by various conditions, such as the host death and different β-lactam antibiotics. Our strategy holds great potential for noninvasive and real-time assessing of the metabolic activities and locations of the highly dynamic gut microbiota.
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