亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Houpo paiqi mixture promotes intestinal motility in constipated rats by modulating gut microbiota and activating 5-HT-cAMP-PKA signal pathway

运动性 阿克曼西亚 内科学 莫沙比利 普雷沃菌属 肠道菌群 肠易激综合征 乳酸菌 洛哌丁胺 便秘 内分泌学 化学 药理学 胃肠病学 生物 医学 生物化学 腹泻 细菌 细胞生物学 发酵 遗传学
作者
Ruixin Wang,Xiaofang Lu,Luqing Zhao,Weixi Zhang,Shengsheng Zhang
出处
期刊:Journal of Applied Microbiology [Wiley]
卷期号:134 (8) 被引量:6
标识
DOI:10.1093/jambio/lxad153
摘要

Constipation is a common functional gastrointestinal disorder, which needs more effective treatment approaches. Houpo Paiqi Mixture (HPPQM) is a type of Chinese patent medicine developed from a classical formula that has been widely applied to the treatment of intestinal motility disorder. Here we aim to assess the effectiveness of HPPQM in the treatment of constipation in rat models and its potential mechanism.UPLC-MS/MS was performed to investigate the chemical component of HPPQM. Rats were randomly divided into normal control, constipation model (CM), HPPQM (low, middle and high dose) and mosapride groups. Loperamide 8 mg/kg was given orally to induce CM. The small intestine motility, colonic contraction, rectum propulsion, and histological feature of the colon were significantly improved in HPPQM group, compared with CM group (P < 0.05). Results of 16S rRNA sequencing revealed that HPPQM treatment strikingly restructured intestinal microbiota in constipated rats by increasing the relative abundances of Bacteroides and Akkermansia and decreasing the relative abundances of Prevotella and Lactobacillus. The levels of GPR43, 5-HT, 5-HT4R, cAMP, PKA were decreased while SERT was increased in constipated rats (P < 0.05), which could be restored to normal levels by treatment with HPPQM (P < 0.05). Differences in amplitude between experimental CLSMs (with HPPQM added) and control CLSMs were discovered, starting at the concentration of 40 nL/mL (P < 0.05). It was found that GLPG0974 and GR113808 could significantly reduce this reactivity (P < 0.05).HPPQM manifested a curative effect in constipated rats by promoting intestinal motility. The underlying mechanisms might be related to modulating gut microbiota and activating 5-HT-cAMP-PKA signal pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小马甲应助科研通管家采纳,获得10
16秒前
16秒前
18秒前
192724836发布了新的文献求助10
33秒前
41秒前
Easypass完成签到 ,获得积分10
43秒前
慕青应助dihaha采纳,获得10
50秒前
烟花应助Leo采纳,获得10
1分钟前
dihaha完成签到,获得积分10
1分钟前
Herbs完成签到 ,获得积分10
1分钟前
FashionBoy应助dihaha采纳,获得10
1分钟前
zqq完成签到,获得积分0
1分钟前
脑洞疼应助十三采纳,获得10
1分钟前
192724836完成签到,获得积分20
1分钟前
小锤发布了新的文献求助10
1分钟前
含蓄的寄翠完成签到,获得积分10
1分钟前
科研通AI2S应助192724836采纳,获得10
1分钟前
tinyliiyong完成签到,获得积分10
1分钟前
1分钟前
sss完成签到 ,获得积分10
1分钟前
小锤完成签到 ,获得积分20
2分钟前
李爱国应助科研通管家采纳,获得10
2分钟前
2分钟前
2分钟前
彭于晏应助十三采纳,获得10
2分钟前
布同完成签到,获得积分10
2分钟前
2分钟前
林思完成签到,获得积分10
2分钟前
老王家的二姑娘完成签到 ,获得积分10
2分钟前
平常的长颈鹿完成签到,获得积分10
2分钟前
852应助平常的长颈鹿采纳,获得10
3分钟前
3分钟前
FashionBoy应助KSung采纳,获得10
3分钟前
3分钟前
小胖子发布了新的文献求助10
3分钟前
机灵自中发布了新的文献求助50
3分钟前
机灵自中完成签到,获得积分10
3分钟前
3分钟前
动人的笑白完成签到,获得积分20
3分钟前
KSung发布了新的文献求助10
3分钟前
高分求助中
Sustainability in ’Tides Chemistry 2000
Studien zur Ideengeschichte der Gesetzgebung 1000
The ACS Guide to Scholarly Communication 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Handbook of the Mammals of the World – Volume 3: Primates 805
Ethnicities: Media, Health, and Coping 800
Gerard de Lairesse : an artist between stage and studio 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3072598
求助须知:如何正确求助?哪些是违规求助? 2726326
关于积分的说明 7493683
捐赠科研通 2374098
什么是DOI,文献DOI怎么找? 1258887
科研通“疑难数据库(出版商)”最低求助积分说明 610394
版权声明 596983