冈田酸
电化学发光
纳米团簇
纳米颗粒
化学
铜
复合数
纳米技术
材料科学
色谱法
检出限
生物化学
有机化学
磷酸酶
复合材料
磷酸化
作者
Xi Chen,Xinyan Zhang,Jinjin Wang,Jie Wei,Qingmin Chen,Xi Chen,Quansheng Chen,Xiaomei Chen
标识
DOI:10.1016/j.foodchem.2024.139844
摘要
In this study, a sensitive dual-signal electrochemiluminescence (ECL) immunosensor was developed for okadaic acid (OA) detection utilizing copper nanoclusters (CuNCs) and Ru(bpy)32+-doped silica nanoparticles (RuSiNPs). Interestingly, the CuNCs could simultaneously enhance both cathodic (−0.95 V) and anodic (+1.15 V) ECL signals of RuSiNPs, forming a dual-signal ECL sensing platform. Further, RuSiNPs@CuNCs were used as immunomarkers by covalently conjugating them with an anti-OA monoclonal antibody (mAb) to form probes. Finally, dual ECL signals of the immunosensor were fabricated and showed good linear relationships with OA concentrations in the range of 0.05–70 ng mL−1, having a median inhibitory concentration (IC50) of 1.972 ng mL−1 and a limit of detection of 0.039 ng mL−1. Moreover, the constant ratio of the cathodic and anodic ECL peaks achieved self-calibration of the detection signal and improved the reliability of the results. Finally, we successfully applied the ECL sensor to detect OA in spiked oyster samples.
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