The freezability of Mediterranean buffalo sperm is associated with lysine succinylation and lipid metabolism

琥珀酰化 精子 赖氨酸 六烯酸 生物 男科 精液 生物化学 化学 脂肪酸 氨基酸 多不饱和脂肪酸 植物 遗传学 医学
作者
Xi Luo,Shihai Huang,Mingming Liang,Qingsong Xue,Saif ur Rehman,Xuan Ren,Yanfang Li,Ting Yang,Deshun Shi,Xiangping Li
出处
期刊:The FASEB Journal [Wiley]
卷期号:36 (12) 被引量:4
标识
DOI:10.1096/fj.202201254r
摘要

Semen cryopreservation is used for the propagation of variety among species and domestic breeding. Mitochondria are implicated in sperm freezability, and their proteins are prone to succinylation, but the relationship between sperm freezability and mitochondrial protein succinylation is unclear. In this study, six bulls were classified as having good or poor freezability ejaculates (GFE or PFE, each 3 bulls). The fresh sperm mitochondrial membrane potential (MMP) and pan succinylation level of the two groups were first detected. Then the lysine succinylome and fatty acid content of the two groups were analyzed using label-free LC–MS/MS and GC–MS/MS in multiple reaction monitoring (MRM) modes, respectively. The results indicated that the GFE sperm had significantly higher MMPs than the PFE group (p < 0.05). A total of 1393 succinylation sites corresponding to 426 proteins were assessed and 5 succinylated peptides of the GFE group were markedly upregulated, while 3 were significantly downregulated (FC > 2.0 – < 0.5 and p-value < 0.05) when compared to the PFE group. Forty-six succinylated proteins were identified to have consistent presence/absence expression. The upregulated succinylated proteins in the GFE sperm were enriched in lipid metabolic processes. A total of 31 fatty acids were further subjected to quantitative analysis of which 23 including arachidic (C20:0), linolenic (C18:3n3), and docosahexaenoic acids (C22:6n3) were decreased in GFE sperm when compared with PFE (p < 0.05). These results suggest that lysine succinylation can potentially influence the sperm freezability of Mediterranean buffaloes through mitochondrial lipid metabolism. This novel study provides our understanding of sperm succinylation and the molecular basis for the mechanism of sperm freezability.
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