Polymorphisms in P53 and VEGFA genes in different subtypes of periorbital hyperpigmentation in a Malaysian Chinese population

Abstract Background/Objectives The unknown pathogenesis of periorbital hyperpigmentation makes its treatment difficult. Existing evidence links p53 and VEGFA genes with skin hyperpigmentation. This study was aimed at (i) identifying the clinical pattern of periorbital hyperpigmentation; and (ii) detecting the presence of VEGFA and P53 single nucleotide polymorphism ( SNP s) in different subtypes of periorbital hyperpigmentation in Malaysian Chinese. Methods A cross‐sectional study was conducted among Malaysian Chinese. Clinical assessments were performed, and medical history was collected. Three regions of p53 and two of VEGFA were amplified by PCR followed by direct sequencing using saliva‐extracted DNA . Results Eighty‐four participants were recruited (average age 22.2 years). In the majority ( n = 62), both eyelids were affected. Facial pigmentary, demarcation lines, tear trough and eye bags were not observed. Mixed (pigmented–vascular) was the most common subtype. Thirteen SNP s were found, nine of which are new. Only three out of 13 SNP s showed significant association with periorbital hyperpigmentation presentation. TA genotype in rs1437756379 ( p53 ) was significantly more prevalent among participants with mixed subtype ( P = 0.011) while AC genotype in rs1377053612 ( VEGFA ) was significantly more prevalent among pigmented subtype ( P = 0.028). AA genotype in rs1479430148 ( VEGFA ) was significantly associated with allergic rhinitis in mixed subtype ( P = 0.012). Conclusion Mixed subtype was the most prevalent type of periorbital hyperpigmentation in the study population. Three polymorphisms in p53 and VEGFA genes were statistically linked with different clinical presentations of periorbital hyperpigmentation.