Effects of titanium dioxide nanoparticles on Microcystis aeruginosa and microcystins production and release

Due to growing production and use, release of nanoparticles (NPs) into the aquatic environment may pose a hazard to ecosystem. In this study, Microcystis aeruginosa was exposed to different concentrations (0.1, 1, 10, 50, 100 mg/L) of titanium dioxide (TiO2) NPs to assess their impact on algae. Meanwhile, the production and release of microcystins (MCs) was determined. Results showed that TiO2 NPs significantly decreased the maximal photochemical efficiency of photosystem II, and thus inhibited the photosynthetic activity of M. aeruginosa. They also increased the content of reactive oxygen species (ROS) and malondialdehyde (MDA), indicating their oxidative damage on algae. Besides, TiO2 NPs at high concentrations (50 and 100 mg/L) aggregated on the algal surface and block the light, herein inhibited algae growth (16.03%±2.50% and 54.13%±0.93%) but induced the production (25.02%±1.23% and 114.43%±2.96%) and release (20.96%±13.30% and 12.10%±8.80%) of MCs. These results indicated that high concentrations of TiO2 NPs increased MCs concentration in water system, which may be harmful to aquatic ecosystem.