纳米材料
纳米颗粒
免疫分析
兴奋剂
背景(考古学)
电化学发光
微乳液
化学
分析物
材料科学
纳米技术
色谱法
光电子学
肺表面活性物质
检出限
生物
生物化学
古生物学
抗体
免疫学
作者
Alessandra Zanut,Francesco Palomba,Matilde Rossi Scota,Sara Rebeccani,Massimo Marcaccio,Damiano Genovese,Enrico Rampazzo,Giovanni Valenti,Francesco Paolucci,Luca Prodi
标识
DOI:10.1002/anie.202009544
摘要
Abstract The combination of highly sensitive techniques such as electrochemiluminescence (ECL) with nanotechnology sparked new analytical applications, in particular for immunoassay‐based detection systems. In this context, nanomaterials, particularly dye‐doped silica nanoparticles (DDSNPs) are of high interest, since they can offer several advantages in terms of sensitivity and performance. In this work we synthesized two sets of monodispersed and biotinylated [Ru(bpy) 3 ] 2+ ‐doped silica nanoparticles, named bio‐Triton@RuNP and bio‐Igepal@RuNP, obtained following the reverse microemulsion method using two different types of nonionic surfactants. Controlling the synthetic procedures, we were able to obtain nanoparticles (NPs) offering highly intense signal, using tri‐ n ‐propylamine (TPrA) as coreactant, with bio‐Triton@RuNps being more efficient than bio‐Igepal@RuNP.
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