Effects of dietary herbal formulae combined by Astragalus polysaccharides, chlorogenic acid and allicin in different combinations and proportions on growth performance, non-specific immunity, antioxidant status, vibriosis resistance and damage indexes of

Aquaculture ResearchVolume 49, Issue 2 p. 701-716 ORIGINAL ARTICLE Effects of dietary herbal formulae combined by Astragalus polysaccharides, chlorogenic acid and allicin in different combinations and proportions on growth performance, non-specific immunity, antioxidant status, vibriosis resistance and damage indexes of Litopenaeus vannamei Hui Huang, Hui Huang The Key Laboratory of Mariculture Ministry of Education, Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorLuqing Pan, Corresponding Author Luqing Pan panlq@ouc.edu.cn orcid.org/0000-0003-4895-9571 The Key Laboratory of Mariculture Ministry of Education, Ocean University of China, Qingdao, Shandong, China Correspondence Luqing Pan, Fisheries College, Ocean University of China, Qingdao, China. Email: panlq@ouc.edu.cnSearch for more papers by this authorShanshan Pan, Shanshan Pan The Key Laboratory of Mariculture Ministry of Education, Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorMengsi Song, Mengsi Song The Key Laboratory of Mariculture Ministry of Education, Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this author Hui Huang, Hui Huang The Key Laboratory of Mariculture Ministry of Education, Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorLuqing Pan, Corresponding Author Luqing Pan panlq@ouc.edu.cn orcid.org/0000-0003-4895-9571 The Key Laboratory of Mariculture Ministry of Education, Ocean University of China, Qingdao, Shandong, China Correspondence Luqing Pan, Fisheries College, Ocean University of China, Qingdao, China. Email: panlq@ouc.edu.cnSearch for more papers by this authorShanshan Pan, Shanshan Pan The Key Laboratory of Mariculture Ministry of Education, Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorMengsi Song, Mengsi Song The Key Laboratory of Mariculture Ministry of Education, Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this author First published: 26 September 2017 https://doi.org/10.1111/are.13500Citations: 11Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract In this study, a feeding trial followed by a challenge test was performed to evaluate effects of six herbal formulae which were different combinations of Astragalus polysaccharides (APS), chlorogenic acid (CGA) and allicin on growth performance, non-specific immune response, antioxidant capacity, disease resistance and biomolecule damage of white shrimp Litopenaeus vannamei. Shrimp were fed seven diets, control diet (basal diet with no herbal formulae) and six herbal formula diets (G1–G6, basal diet supplemented with 0.1% APS + 0.05% allicin, 0.1% APS + 0.1% allicin, 0.1% CGA + 0.05% allicin, 0.1% CGA + 0. 1% allicin, 0.1% APS + 0.1% CGA and 0.1% APS + 0.1% CGA + 0.05% allicin respectively), for 21 days. After that, shrimp were challenged with Vibrio harveyi and then the cumulative mortality of shrimp was recorded for 7 days post challenge. The results showed that there were no significant differences in growth performance among all groups, while the non-specific immune responses and antioxidant indexes were significantly improved (p < .05) in shrimp fed herbal formula diets when compared to the control. Meanwhile, the lowest cumulative mortality was observed in shrimp fed herbal formula diets with 0.1% APS, 0.1% CGA and 0.05% allicin supplementation after V. harveyi challenge. Additionally, herbal formulae could not cause biomolecule damage to the hepatopancreas of shrimp. In conclusion, these results indicated that synergistic effect of APS, CGA and allicin helped to boost immunity, antioxidant capacity and disease resistance of shrimp without biomolecule damage. Citing Literature Volume49, Issue2February 2018Pages 701-716 RelatedInformation