亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Intra-articular Recombinant Human Proteoglycan 4 Mitigates Cartilage Damage After Destabilization of the Medial Meniscus in the Yucatan Minipig

医学 滑液 软骨 关节软骨损伤 内侧半月板 骨关节炎 蛋白多糖 生理盐水 弯月面 阿格里坎 糖胺聚糖 透明质酸 病理 关节软骨 内科学 解剖 物理 替代医学 入射(几何) 光学
作者
Kimberly A. Waller,Kaitlyn E. Chin,Gregory D. Jay,Ling X. Zhang,Erin Teeple,Scott McAllister,Gary J. Badger,Tannin A. Schmidt,Braden C. Fleming
出处
期刊:American Journal of Sports Medicine [SAGE]
卷期号:45 (7): 1512-1521 被引量:72
标识
DOI:10.1177/0363546516686965
摘要

Lubricin, or proteoglycan 4 (PRG4), is a glycoprotein responsible for joint boundary lubrication. PRG4 has been shown previously to be down-regulated after traumatic joint injury such as a meniscal tear. Preliminary evidence suggests that intra-articular injection of PRG4 after injury will reduce cartilage damage in rat models of surgically induced posttraumatic osteoarthritis.To determine the efficacy of intra-articular injection of full-length recombinant human lubricin (rhPRG4) for reducing cartilage damage after medial meniscal destabilization (DMM) in a preclinical large animal model.Controlled laboratory study.Unilateral DMM was performed in 29 Yucatan minipigs. One week after DMM, animals received 3 weekly intra-articular injections (3 mL per injection): (1) rhPRG4 (1.3 mg/mL; n = 10); (2) rhPRG4+hyaluronan (1.3 mg/mL rhPRG4 and 3 mg/mL hyaluronan [~950 kDA]; n = 10); and (3) phosphate-buffered saline (PBS; n = 9). Hindlimbs were harvested 26 weeks after surgery. Cartilage integrity was evaluated by use of macroscopic (India ink) and microscopic (safranin O-fast green and hematoxylin and eosin) scoring systems. Secondary outcomes evaluated via enzyme-linked immunosorbent assay (ELISA) included PRG4 levels in synovial fluid, carboxy-terminal telepeptide of type II collagen (CTX-II) concentrations in urine and serum, and interleukin 1β (IL-1β) levels in synovial fluid and serum.The rhPRG4 group had significantly less macroscopic cartilage damage in the medial tibial plateau compared with the PBS group ( P = .002). No difference was found between the rhPRG4+hyaluronan and PBS groups ( P = .23). However, no differences in microscopic damage scores were observed between the 3 groups ( P = .70). PRG4 production was elevated in the rhPRG4 group synovial fluid compared with the PBS group ( P = .033). The rhPRG4 group presented significantly lower urinary CTX-II levels, but not serum levels, when compared with the PBS ( P = .013) and rhPRG4+hyaluronan ( P = .011) groups. In serum and synovial fluid, both rhPRG4 ( P = .006; P = .017) and rhPRG4+hyaluronan groups ( P = .009; P = .03) presented decreased IL-1β levels.All groups exhibited significant cartilage degeneration after DMM surgery. However, animals treated with rhPRG4 had the least amount of cartilage damage and less inflammation, providing evidence that intra-articular injections of rhPRG4 may slow the progression of posttraumatic osteoarthritis.Patients with meniscal trauma are at high risk for posttraumatic osteoarthritis. This study demonstrates that an intra-articular injection regimen of rhPRG4 may attenuate cartilage damage after meniscal injury.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
隐形曼青应助好运来采纳,获得10
1秒前
fantw发布了新的文献求助10
7秒前
所所应助易xuan采纳,获得10
7秒前
zyj完成签到 ,获得积分10
10秒前
zyj关注了科研通微信公众号
15秒前
楚狂接舆完成签到,获得积分10
19秒前
22秒前
23秒前
晚来天欲雪完成签到 ,获得积分10
24秒前
25秒前
哦呦呦发布了新的文献求助10
25秒前
32秒前
39秒前
星辰大海应助xiyu采纳,获得10
57秒前
58秒前
易xuan完成签到,获得积分10
59秒前
1分钟前
易xuan发布了新的文献求助10
1分钟前
科研通AI2S应助科研通管家采纳,获得10
1分钟前
1分钟前
1分钟前
模糊中正应助村雨采纳,获得30
1分钟前
1分钟前
big ben完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
xiyu发布了新的文献求助10
1分钟前
1分钟前
xiyu完成签到,获得积分10
1分钟前
mini的yr完成签到 ,获得积分10
1分钟前
辣椒完成签到 ,获得积分10
1分钟前
Murphy完成签到 ,获得积分10
1分钟前
2分钟前
2分钟前
2分钟前
senger发布了新的文献求助10
2分钟前
勿昂完成签到 ,获得积分10
2分钟前
2分钟前
2分钟前
高分求助中
Natural History of Mantodea 螳螂的自然史 1000
进口的时尚——14世纪东方丝绸与意大利艺术 Imported Fashion:Oriental Silks and Italian Arts in the 14th Century 800
Glucuronolactone Market Outlook Report: Industry Size, Competition, Trends and Growth Opportunities by Region, YoY Forecasts from 2024 to 2031 800
A Photographic Guide to Mantis of China 常见螳螂野外识别手册 800
Zeitschrift für Orient-Archäologie 500
Smith-Purcell Radiation 500
Autoregulatory progressive resistance exercise: linear versus a velocity-based flexible model 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3344111
求助须知:如何正确求助?哪些是违规求助? 2971140
关于积分的说明 8646622
捐赠科研通 2651377
什么是DOI,文献DOI怎么找? 1451711
科研通“疑难数据库(出版商)”最低求助积分说明 672250
邀请新用户注册赠送积分活动 661788