张力素
PTEN公司
小RNA
微泡
外体
癌症研究
癌症
逆转录聚合酶链式反应
内科学
生物
医学
分子生物学
信使核糖核酸
细胞凋亡
生物化学
基因
PI3K/AKT/mTOR通路
作者
Sıdıka Genç,Tarık Yağcı,Dimitra P. Vageli,Rıza Dündar,Panagiotis G. Doukas,Sotirios G. Doukas,Maria Tolia,Nikolaos Chatzakis,Aristidis Tsatsakis,Ali Taghizadehghalehjoughi
出处
期刊:ACS pharmacology & translational science
[American Chemical Society]
日期:2023-04-24
卷期号:6 (5): 820-828
被引量:4
标识
DOI:10.1021/acsptsci.3c00038
摘要
Laryngeal squamous cell carcinoma (LSCC) is one of the most aggressive cancers, and its early diagnosis is urgent. Exosomes are believed to have diagnostic significance in cancer. However, the role of serum exosomal microRNAs, miR-223, miR-146, and miR-21, and phosphatase and tensin homologue (PTEN) and hemoglobin subunit delta (HBD) mRNAs in LSCC is unclear. Exosomes were isolated from the blood serum of 10 LSCC patients and 10 healthy controls to perform scanning electron microscopy and liquid chromatography quadrupole time-of-flight mass spectrometry analyses to characterize them and to undergo reverse transcription polymerase chain reaction to identify miR-223, miR-146, miR-21, and PTEN and HBD mRNA expression phenotypes. Biochemical parameters, including serum C-reactive protein (CRP) and vitamin B12, were also obtained. Serum exosomes of 10–140 nm were isolated from LSCC and controls. Serum exosomal miR-223, miR-146, and PTEN were found to be significantly decreased (p < 0.05), in contrast to serum exosomal miRNA-21 (p < 0.01), and serum vitamin B12 and CRP (p < 0.05) were found to be significantly increased, in LSCC vs controls. Our novel data show that the combination of reduced serum exosomal miR-223, miR-146, and miR-21 profiles and biochemical alterations in CRP and vitamin B12 levels may be useful indicators of LSCC that could be validated by large studies. Our findings also suggest a possible negative regulatory effect of miR-21 on PTEN in LSCC, encouraging a more extensive investigation of its role.
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