Portable smartphone platform based on a ratio fluorescence probe for situ visual monition of cardiac disease markers in vitro

荧光 生物相容性 检出限 发光 离解(化学) 肉眼 荧光寿命成像显微镜 化学 纳米技术 材料科学 分析化学(期刊) 光化学 生物物理学 光电子学 色谱法 生物 光学 物理化学 有机化学 物理
作者
Shanshan Wei,Hongyuan Zhang,Chenzhao Wang,Xiangyu Yin,Kai-Xin Hu,Min Liu,Chunzhu Jiang,Guoying Sun
出处
期刊:Chemical Engineering Journal [Elsevier BV]
卷期号:474: 145614-145614 被引量:19
标识
DOI:10.1016/j.cej.2023.145614
摘要

Creatine kinase (CK), as an important biochemical index of cardiac injury, is very significant for early and accurate diagnosis of acute myocardial infarction or cardiac arrest. However, the sensitivity and specificity of CK detection still face great challenges. Herein, a ratio fluorescence probe was designed and prepared by electrostatic interaction between boron and nitrogen doped carbon dots (BN-L-CDs) as a response signal and Ru(bpy)3Cl2·6H2O as independent reference signal. Notably, the BN-L-CDs@[Ru(bpy)3]2+ displayed an ultra-fast fluorescence response to CK in ATP medium and an excellent linear relationship was observed between the concentration of CK and the fluorescence recovery ratio with a detection limit as low as 1.20 U/L. Mechanism investigates demonstrated that the "ON-OFF-ON" progress could be attributed to the formation of the non-luminescent ground state complex BN-L-CDs-ATP by the interaction of BN-L-CDs with ATP and the dissociation of complex under the catalysis of CK, respectively. Besides, the ratio fluorescence probe was further applied to living cell imaging due to its excellent biocompatibility. Importantly, a portable visual sensing platform was developed by combining a ratio fluorescence probe with a mobile phone color recognition application. Therefore, this new sensor provided a new perspective for the convenient monitoring of CK and paved the way for the relevant applications of clinical diagnostics.
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