类有机物
诱导多能干细胞
胚状体
再生医学
定向微分
干细胞
生物
细胞生物学
人诱导多能干细胞
体细胞
解剖
胚胎干细胞
遗传学
基因
作者
I. Ahmed,Jane Sun,Jason Brown,Kiarash Khosrotehrani,Abbas Shafiee
标识
DOI:10.1093/biomethods/bpae019
摘要
Abstract Organoid generation from pluripotent stem cells is a cutting-edge technique that has created new possibilities for modelling human organs in vitro, as well as opening avenues for regenerative medicine. Here, we present a protocol for generating skin organoids (SKOs) from human-induced pluripotent stem cells (hiPSCs) via direct embryoid body formation. This method provides a consistent start point for hiPSC differentiation, resulting in SKOs with complex skin architecture and appendages (e.g. hair follicles, sebaceous glands, etc.) across hiPSC lines from two different somatic sources.
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