互补DNA
肽序列
生物化学
氨基酸
核酸序列
分子生物学
芳香族L-氨基酸脱羧酶
cDNA文库
生物
磷酸吡哆醛
酶
寡核苷酸
克隆(Java方法)
蛋白质一级结构
基因
化学
辅因子
作者
Hiroshi Ichinose,Yoshikazu Kurosawa,K Titani,Keisuke Fujita,Toshiharu Nagatsu
标识
DOI:10.1016/0006-291x(89)91772-5
摘要
The nucleotide sequence of a cDNA clone that includes the entire coding region of human aromatic L-amino acid decarboxylase gene is presented. A human pheochromocytoma cDNA library was screened using an oligonucleotide probe which corresponded to a partial amino acid sequence of the enzyme purified from the human pheochromocytoma. The isolated cDNA clone encoded a protein of 480 amino acids with a calculated molecular mass of 53.9 kDa. The amino acid sequence Asn-Phe-Asn-Pro-His-Lys-Trp around a possible cofactor (pyridoxal phosphate) binding site is identical in human, Drosophila, and pig enzymes.
科研通智能强力驱动
Strongly Powered by AbleSci AI