球孢白僵菌
生物
转化(遗传学)
可选择标记
毒力
球孢菌
农杆菌
遗传学
基因
根癌农杆菌
突变体
植物
生物病虫害防治
作者
Weiguo Fang,Yongjun Zhang,Xingyong Yang,Xuelian Zheng,Hui Duan,Yi Li,Yan Pei
标识
DOI:10.1016/j.jip.2003.12.003
摘要
Beauveria bassiana has been investigated for use in the biological control of several insects in agricultural practice. To understand the molecular basis of virulence and host specificity and to improve the entomopathogenicity of B. bassiana, we have developed a simple, highly efficient and reliable Agrobacterium-mediated transformation method for B. bassiana using a phosphinothricin acetyltransferase (bar) gene as a selectable marker. Most transformants contained single copies of T-DNA and the T-DNA inserts were stably inherited after five generations. With this highly efficient transformation method for B. bassiana, we also obtained two putative T-DNA-tagged mutants that may have altered growth habits or virulence. Thus, the described protocol could provide a useful tool to manipulate the genetic make-up and to tag genes that may be important for virulence or growth and development of B. bassiana.
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