核黄素
乳酸乳球菌
代谢工程
生物化学
生产过剩
维生素
B族维生素
精氨酸脱氨酶
化学
生物
食品科学
细菌
酶
精氨酸
遗传学
氨基酸
乳酸
内分泌学
作者
Wilbert Sybesma,Catherine M. Burgess,Marjo Starrenburg,Douwe van Sinderen,Jeroen Hugenholtz
标识
DOI:10.1016/j.ymben.2003.11.002
摘要
The dairy starter bacterium Lactococcus lactis has the potential to synthesize both folate (vitamin B11) and riboflavin (vitamin B2). By directed mutagenesis followed by selection and metabolic engineering we have modified two complicated biosynthetic pathways in L. lactis resulting in simultaneous overproduction of both folate and riboflavin: Following exposure to the riboflavin analogue roseoflavin we have isolated a spontaneous mutant of L. lactis strain NZ9000 that was changed from a riboflavin consumer into a riboflavin producer. This mutant contained a single base change in the regulatory region upstream of the riboflavin biosynthetic genes. By the constitutive overproduction of GTP cyclohydrolase I in this riboflavin-producing strain, the production of folate was increased as well. Novel foods, enriched through fermentation using these multivitamin-producing starters, could compensate the B-vitamin-deficiencies that are common even in highly developed countries and could specifically be used in dietary foods for the large fraction of the Caucasian people (10-15%) with mutations in the methylene tetrahydrofolate reductase (MTHFR).
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