细胞毒性
咔唑
烷基
化学
立体化学
流式细胞术
K562细胞
吲哚试验
生物碱
细胞凋亡
生物化学
生物
有机化学
分子生物学
体外
作者
C. Díez,Mariela Céspedes,Gabriela Mariana Di Venosa,Gustavo Calvo,Esteban Avigliano,Adriana Casas,Jorge Alejandro Palermo
标识
DOI:10.1080/14786419.2021.1880401
摘要
In this work, eleven new derivatives were prepared of the alkaloid olivacine (1), which was isolated from the bark of Aspidosperma australe. These compounds (7a-k) are hybrids of olivacine and indoles or carbazole, tethered by alkyl chains of variable lengths (C-4, C-5 or C-6). Compounds 7a-k showed increased cytotoxicity towards a panel of four cell lines. The subcellular localization of olivacine and of the synthetic derivatives was studied by fluorescence microscopy. The cycles of K562 cells exposed to olivacine or compounds 7a-k were analysed by flow cytometry, and showed, for some of the new derivatives, a different profile of cell distribution among the phases of the cycle when compared to olivacine, which is indicative of lysosomal apoptosis.
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