生物
基因
基因组
基因组编辑
计算生物学
遗传学
功能(生物学)
作者
Xin Wang,Lingling Ye,Munan Lyu,Robertas Ursache,Ari Löytynoja,Ari Pekka Mähönen
出处
期刊:Nature plants
[Nature Portfolio]
日期:2020-06-29
卷期号:6 (7): 766-772
被引量:90
标识
DOI:10.1038/s41477-020-0695-2
摘要
Conditional manipulation of gene expression is a key approach to investigating the primary function of a gene in a biological process. While conditional and cell-type-specific overexpression systems exist for plants, there are currently no systems available to disable a gene completely and conditionally. Here, we present a new tool with which target genes can efficiently and conditionally be knocked out by genome editing at any developmental stage. Target genes can also be knocked out in a cell-type-specific manner. Our tool is easy to construct and will be particularly useful for studying genes having null alleles that are non-viable or show pleiotropic developmental defects.
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