Microbial fuel cells (MFCs) are bioelectrochemical devices that produces electric power while treating wastewaters. These unique systems relies on the ability of certain electroactive bacteria to transfer their electrons extracellulary as a part of their metabolism when placed in proximity to a solid electrode. In recent years, the research interst in these systems have grown tremendously and the first commercial prototypes are expected soon. This chapter presents an introduction to the MFC technology, its working principle, common measurement techniques, the basic design and structure of the cell, electron transfer mechanism and some proven and potential applications.