癌症研究
小RNA
下调和上调
细胞迁移
甲状腺癌
转化生长因子
转化生长因子β
上皮-间质转换
细胞生长
庆大霉素保护试验
细胞
基质金属蛋白酶
生物
化学
癌症
转移
内科学
细胞生物学
医学
内分泌学
甲状腺
基因
生物化学
遗传学
作者
Zhihong Wang,Hao Zhang,Ping Zhang,Wenwu Dong,Liang He
出处
期刊:Tumor Biology
[SAGE]
日期:2015-12-19
卷期号:37 (6): 7633-7644
被引量:29
标识
DOI:10.1007/s13277-015-4653-y
摘要
MicroRNA-663 (miR-663) has been detected in a large variety of tumor types; however, it still holds both tumor suppressive and oncogenic roles in different tumor types. The miRNA-CHIP microarray assay revealed downregulation of miR-663 in papillary thyroid carcinoma (PTC); however, the effect of miR-663 on PTC cell invasion and migration remains unknown. Accordingly, this study aimed to investigate the potential involvement of miR-663 in PTC. In this study, miR-663 expression level was measured via quantitative real-time PCR in 91 pairs of human PTC and adjacent normal tissues and in two human PTC cell lines. The effect of miR-663 on PTC cell invasion and migration were studied by transwell and wound healing assays. In addition, the miR-663 target was searched and the underlying mechanism was clarified by reporter assay and rescue experiment. The current study confirmed that miR-663 expression was inhibited in PTC tissue samples and PTC cell lines. There were statistically significant differences in expression of miR-663 with regard to age and tumor size. Upregulation of miR-663 suppressed PTC cell invasion and migration. Further study showed that transforming growth factor beta 1 (TGFβ1) was the direct target of miR-663 and mediated the effect of miR-663 on PTC development. By targeting TGFβ1, miR-663 efficiently regulates the expression of epithelial-mesenchymal transition (EMT) markers and matrix metalloproteinases (MMPs). The data indicated that miR-663 may suppress tumor invasion and migration by targeting TGFβ1 and regulate EMT progress of PTC cells.
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