生物
16S核糖体RNA
拉伤
微生物学
植物
生物化学
食品科学
基因
解剖
作者
David Heidler von Heilborn,Lena-Luisa Nover,Mareike Weber,Georg Hölzl,Nicolas Gisch,Claudia Waldhans,Maureen Mittler,Judith Kreyenschmidt,Christian Woehle,Bruno Huettel,André Lipski
标识
DOI:10.1099/ijsem.0.005372
摘要
Modified atmosphere (MA) packaging plays an important role in improving food quality and safety. By using different gas mixtures and packaging materials the shelf life of fresh produce can significantly be increased. A Gram-negative-staining, rod-shaped, orange-pigmented strain DH-B6 T , has been isolated from MA packed raw pork sausage (20% CO 2 , 80% O 2 ). The strain produced biofilms and showed growth at high CO 2 levels of up to 40%. Complete 16S rRNA gene and whole-genome sequences revealed that strain DH-B6 T belongs to the genus Chryseobacterium , being closely related to strain Chryseobacterium indologenes DSM 16777 T (98.4%), followed by Chryseobacterium gleum NCTC11432 T (98.3%) and Chryseobacterium lactis KC1864 T (98.2%). Average nucleotide identity value between DH-B6 T and C. indologenes DSM 16777 T was 81.1% and digital DNA–DNA hybridisation was 24.9%, respectively. The DNA G+C content was 35.51 mol%. Chemotaxonomical analysis revealed the presence of the rare glycine lipid cytolipin, the serine-glycine lipid flavolipin and the sulfonolipid sulfobacin A, as well as phosphatidylethanolamine, monohexosyldiacylglycerol and ornithine lipid, including the hydroxylated forms. Major fatty acids were iC 15 : 0 (50.7%) and iC 17 : 1 cis 9 (28.7%), followed by iC 15 : 0 2-OH (7.0%) and iC 17 : 0 3-OH (6.2%). The isolated strain contained MK-6 as the only respiratory quinone and flexirubin-like pigments were detected as the major pigments. Based on the phenotypic, chemotaxonomic and phylogenetic characteristics, the strain DH-B6 T (=DSM 110542 T =LMG 31915 T ) represents a novel species of the genus Chryseobacterium , for which the name Chryseobacterium capnotolerans sp. nov. is proposed. Emended descriptions of the genus Chryseobacterium and eight species of this genus based on polar lipid characterisation are also proposed.
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