Anaerobic spectrophotometry: An improved cuvette

A cuvette is described which allows purging of the reaction solution with any gas mixture. The cuvette design incorporates a gas inlet and outlet which can be simultaneously sealed by a small angular rotation of the Thunberg head and a gassing tube which is an integral part of the body of the cuvette and which is, therefore, not easily broken. Anaerobic conditions can be achieved in 10 min and can be maintained, in the sealed cuvette, for at least 12 hr.