白藜芦醇
大肠杆菌
异源的
基因
生物
生物化学
生物合成
化学
作者
Xianglei Liu,Jun Lin,Haifeng Hu,Bin Zhou,Zhu Bao-quan
出处
期刊:Fems Microbiology Letters
[Oxford University Press]
日期:2016-03-13
卷期号:363 (8): fnw061-fnw061
被引量:29
标识
DOI:10.1093/femsle/fnw061
摘要
Resveratrol is a well-known triphenolic natural product present in red wine. For its contribution to human health, the demand for resveratrol as a food and nutrition supplement has increased significantly. In recent years, the rapid development of synthetic biology has promoted extensive work to increase the production of resveratrol in microbes. However, supplementation of expensive phenylpropanoic precursors was required in current engineered strains. Here, we first utilized the site-specific integration strategy to produce resveratrol in Escherichia coli The genes tal, 4cl and sts were site-specific integrated into the loci of genes tyrR and trpED in the chromosome of E. coli BW25113 (DE3). The final strain was capable of producing 4.612 mg L(-1) of resveratrol from glucose.
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