[Analysis of coumarins in Fructus Cnidii by HPLC-ESI-MS].

To analyze coumarins in Fructus Cnidii by HPLC-ESI-MS.Coumarins in Fructus Cnidii were subjected to HPLC, using Shim-pack CLC-ODS column, methanol-water (70:30) as mobile phase, and 254 nm as DAD wavelength. A quadrupole mass spectrometer was applied to verify the LC elute, operated in the positive ion mode.Three coumarins were determined and the possible structures of seven coumarins were deduced based on the chromatography and MS data.The application of this method could be available for the rapid identification of constituents from traditional Chinese medicines.