Isolation and identification of chemical constituents from the flowers of Abelmoschus manihot(L.)Medic(III)

Objective To study the chemical constituents from the flowers of Abelmoschus manihot(L.)Medic.Methods The compounds were isolated by chromatographic methods such as silica gel,ODS,Sephadex LH-20 and HPLC,and their structures were identified by physicochemical properties,especially spectral analysis.Results Nine compounds were isolated.The structures of them were identified as gossypetin 8-O-β-D-glucuronide(1),gossypetin 3-O-β-glucopyranoside-8-O-β-glucuronopyranoside(2),gossypetin 3′-O-β-glucopyranoside(3),tiliroside(4),kaempferol 3-O-[3″-O-acetyl-6″-O-(E)-p-coumaroyl)]-β-D-glucopyranoside(5),quercetin 3-O-[β-D-xylopyranosyl(1→2)-α-L-rhamnopyranosyl(1→6)]-β-D-galactopyranoside(6),4-hydroxybenzoic acid β-D-glucose ester(7),protocatechuic acid(8) and protocatecheuic acid-3-O-β-glucoside(9),respectively.Conclusion Compounds 2,4-9 are obtained from Abelmoschus genus for the first time,and the spectral data in DMSO-d6 solution of 5,6 and 9 are reported firstly.