黄曲霉毒素
黄曲霉
麦角甾醇
超氧化物歧化酶
化学
活性氧
生物化学
过氧化氢酶
超氧化物
DNA断裂
抗氧化剂
生物
程序性细胞死亡
酶
食品科学
细胞凋亡
作者
Wei Shan,Qianru Xu,Shan Pei,Yangyong Lv,Yang Lei,Shuaibing Zhang,Huan-Chen Zhai,Yuansen Hu
标识
DOI:10.1016/j.fm.2024.104588
摘要
Aspergillus flavus infects important crops and produces carcinogenic aflatoxins, posing a serious threat to food safety and human health. Biochemical analysis and RNA-seq were performed to investigate the effects and mechanisms of piperitone on A. flavus growth and aflatoxin B1 biosynthesis. Piperitone significantly inhibited the growth of A. flavus, AFB1 production, and its pathogenicity on peanuts and corn flour. Differentially expressed genes (DEGs) associated with the synthesis of chitin, glucan, and ergosterol were markedly down-regulated, and the ergosterol content was reduced, resulting in a disruption in the integrity of the cell wall and cell membrane. Moreover, antioxidant genes were down-regulated, the correspondingly activities of antioxidant enzymes such as catalase, peroxidase, and superoxide dismutase were reduced, and levels of superoxide anion and hydrogen peroxide were increased, leading to a burst of reactive oxygen species (ROS). Accompanied by ROS accumulation, DNA fragmentation and cell autophagy were observed, and 16 aflatoxin cluster genes were down-regulated. Overall, piperitone disrupts the integrity of the cell wall and cell membrane, triggers the accumulation of ROS, causes DNA fragmentation and cell autophagy, ultimately leading to defective growth and impaired AFB1 biosynthesis.
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