FEN1 promotes cancer progression of cholangiocarcinoma by regulating the Wnt/β-catenin signaling pathway

Wnt信号通路 癌症研究 DNA修复 DNA损伤 增殖细胞核抗原 细胞生长 分子生物学 生物 信号转导 细胞生物学 DNA 遗传学
作者
Xie Yuwei,Yangang Wang,Sun Zhaowei,Yujie Feng,Wei Zhao,Kun Li,Kui Liu,Jingyu Cao,Chengzhan Zhu
出处
期刊:Digestive and Liver Disease [Elsevier]
卷期号:56 (4): 695-704 被引量:4
标识
DOI:10.1016/j.dld.2023.08.050
摘要

Purpose Cholangiocarcinoma (CHOL) comprises a cluster of highly heterogeneous malignant biliary tumors. Flap endonuclease-1 (FEN1) is a member of the Rad2 structure-specific nuclease family. This study aimed to explore the biological functions and mechanisms of FEN1 in CHOL. Methods FEN1 expression was analyzed in tissues of patients with CHOL and FEN1 mutations. We observe the influence of FEN1 on cellular proliferation, migration, and invasion, as well as on DNA damage repair and glycolysis. Western blotting was performed to determine the regulatory mechanism of FEN1 in CHOL progression. Results FEN1 was highly expressed in the cancer tissues of CHOL patients. The high mutation rate of FEN1 in CHOL tissues was mainly due to the amplified repeats. FEN1 promotes the proliferation, migration, and invasion of HUCCT1 and QBC939 cells. In addition, FEN1 induced DNA damage repair and aerobic glycolysis in CHOL cells. FEN1 also promoted xenograft tumor growth in vivo. Moreover, we showed that FEN1 mediated the epithelial–mesenchymal transition (EMT) of CHOL. FEN1-mediated EMT was found to be transduced by the Wnt/β-catenin signaling pathway. Conclusion FEN1 was significantly overexpressed in CHOL tissues, and FEN1 regulates the progression of CHOL through the Wnt/β-catenin signaling pathway.
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