生物
扩散
生物系统
反应扩散系统
细胞
细胞生物学
计算生物学
物理
生物化学
数学
数学分析
热力学
作者
Rohith Rajasekaran,Chih‐Chia Chang,Elliott W.Z. Weix,Thomas M. Galateo,Scott M. Coyle
出处
期刊:Cell
[Elsevier]
日期:2024-01-01
卷期号:187 (2): 345-359.e16
标识
DOI:10.1016/j.cell.2023.12.007
摘要
Cells self-organize molecules in space and time to generate complex behaviors, but we lack synthetic strategies for engineering spatiotemporal signaling. We present a programmable reaction-diffusion platform for designing protein oscillations, patterns, and circuits in mammalian cells using two bacterial proteins, MinD and MinE (MinDE). MinDE circuits act like “single-cell radios,” emitting frequency-barcoded fluorescence signals that can be spectrally isolated and analyzed using digital signal processing tools. We define how to genetically program these signals and connect their spatiotemporal dynamics to cell biology using engineerable protein-protein interactions. This enabled us to construct sensitive reporter circuits that broadcast endogenous cell signaling dynamics on a frequency-barcoded imaging channel and to build control signal circuits that synthetically pattern activities in the cell, such as protein condensate assembly and actin filamentation. Our work establishes a paradigm for visualizing, probing, and engineering cellular activities at length and timescales critical for biological function.
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