Noval Selective Ligand‐Extraction of Bioactive Components in Complex Natural Products Applying Immobilized Multi‐Target Magnetic Beads

ABSTRACT Choerospondias axillaris is a medicinal plant used for treating coronary heart disease (CHD) due to its broad spectrum of anti‐inflammatory activities. Cyclooxygenase 2 (COX‐2) and lipoxygenase 5 (5‐LOX) were immobilized on magnetic nanoparticles for selective ligand‐extraction of these two enzymes present in C. axillaris . Sixteen extracted components were identified and analyzed using ultraperformance liquid chromatography plus Q‐Exactive Orbitrap tandem mass spectrometry (UHPLC‐Q‐ExactiveOrbitrap‐MS/MS), including flavonoids Curcumin and Epicatechin. The metabolization of the aforementioned 16 components in rats with acute blood stasis indicated a solid pharmacodynamic foundation. This method offers an effective approach for conducting basic research on the enrichment, analysis, and efficacy evaluation of active components in complex natural products.