铜绿微囊藻
水杨酸
爆发
化学
微生物学
嗜麦芽窄食单胞菌
过氧化氢
蓝藻
生物化学
生物
药理学
铜绿假单胞菌
细菌
病毒学
遗传学
作者
Chen Luo,Chenlan Chen,Xuanxuan Xian,Wei-Feng Cai,Xin Yu,Chengsong Ye
标识
DOI:10.1016/j.jhazmat.2024.134196
摘要
The secondary outbreak of cyanobacteria after algicide treatment has been a serious problem to water ecosystems. Hydrogen peroxide (H2O2) is an algaecide widely used in practice, but similar re-bloom problems are inevitably encountered. Our work found that Microcystis aeruginosa (M. aeruginosa) temporarily hibernates after H2O2 treatment, but there is still a risk of secondary outbreaks. Interestingly, the dormant period was as long as 20 and 28 days in 5 mg L-1 and 20 mg L-1 H2O2 treatment groups, respectively, but the photosynthetic activity was both restored much earlier (within 14 days). Subsequently, a quantitative imaging flow cytometry-based method was constructed and confirmed that the re-bloom had undergone two stages including first recovery and then re-division. The expression of ftsZ and fabZ genes showed that M. aeruginosa had active transcription processes related to cell division protein and fatty acid synthesis during the dormancy stat. Furthermore, metabolomics suggested that the recovery of M. aeruginosa was mainly by activating folate and salicylic acid synthesis pathways, which promoted environmental stress resistance, DNA synthesis, and cell membrane repair. This study reported the comprehensive mechanisms of secondary outbreak of M. aeruginosa after H2O2 treatment. The findings suggest that optimizing the dosage and frequency of H2O2, as well as exploring the potential use of salicylic acid and folic acid inhibitors, could be promising directions for future algal control strategies. The secondary outbreak of cyanobacteria after algicide treatment has been a serious problem in aquatic system. To better understand the mechanism, a laboratory simulation was conducted to observe the entire process of algae disappearing and re-emerging after H2O2 treatment. The results showed that M. aeruginosa cells experienced a process of dormancy - activity recovery - re-division when exposed to a dosage of 5-20 mg L-1. Metabolomics indicated that folate and salicylic acid synthesis were metabolic features of re-blooms. Therefore, it is crucial to consider moderately increasing the dosage of algaecide and implementing regular control measures to effectively manage this issue.
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