秀丽隐杆线虫
生殖系
免疫染色
生物
模式生物
细胞生物学
体细胞
细胞凋亡
突变体
免疫荧光
抗体
分子生物学
遗传学
免疫组织化学
基因
免疫学
作者
Benjamin Lant,W. Brent Derry
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2014-05-01
卷期号:2014 (5): pdb.prot080242-pdb.prot080242
被引量:6
标识
DOI:10.1101/pdb.prot080242
摘要
The transparency of Caenorhabditis elegans makes it an ideal organism for visualizing proteins by immunofluorescence microscopy; however, the tough cuticle of worms and the egg shell surrounding embryos pose challenges in achieving effective fixation so that antibodies can diffuse into cells. In this protocol, we describe immunostaining of apoptosis-related proteins in the C. elegans adult germline using fluorescent reagents. Protein localization and abundance can be determined in various mutant backgrounds and under a variety of conditions, such as exposure to genotoxic stress. The number of antibodies specific to C. elegans proteins is quite limited compared with other organisms, but there is a growing list of immunological reagents directed against proteins in other organisms that cross-react with the homologous C. elegans proteins.
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