病毒学
套式聚合酶链反应
基因组
生物
病毒
聚合酶链反应
遗传学
基因
作者
Marcos Lázaro Moreli,Víctor Hugo Aquino,Ana Cecília Ribeiro Cruz,Luíz Tadeu Moraes Figueiredo
摘要
Abstract Using the RT‐PCR with primers that anneal to the 5′ and the 3′ extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT‐nested‐PCR is a useful tool for rapid diagnosis of Oropouche fever infections. J. Med. Virol. 66:139–142, 2002. © 2002 Wiley‐Liss, Inc.
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