原噬菌体
生物
沙雷菌
微生物学
幼虫
噬菌体
细菌
大肠杆菌
遗传学
植物
基因
假单胞菌
作者
Christon J. Hurst,Sam S. Beard,T.A. Jackson,Sandra M. Jones
出处
期刊:Fems Microbiology Letters
[Oxford University Press]
日期:2007-02-02
卷期号:270 (1): 42-48
被引量:77
标识
DOI:10.1111/j.1574-6968.2007.00645.x
摘要
The Serratia entomophila antifeeding prophage (Afp) is thought to form a virus-like structure that has activity towards the New Zealand grass grub, Costelytra zealandica. Through the trans based expression of AnfA1, an RfaH – like transcriptional antiterminator, the Afp, was able to be induced. The expressed Afp was purified and visualized by electron microscopy. The Afp resembled a phage tail-like bacteriocin, exhibiting two distinct morphologies: an extended and a contracted form. The purified Afp conferred rapid activity towards C. zealandica larvae, causing cessation of feeding and a change to an amber colouration within 48 h postinoculation, with increased dose rates causing larval mortality.
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