拟南芥
冷冲击域
发芽
生物
脱落酸
细胞生物学
赤霉素
核糖核酸
热休克蛋白
分解代谢
核酸
适应
基因
野生型
基因表达
突变体
生物化学
植物
新陈代谢
作者
Kentaro Sasaki,Myung‐Hee Kim,Yuri Kanno,Mitsunori Seo,Yuji Kamiya,Ryozo Imai
标识
DOI:10.1016/j.bbrc.2014.11.092
摘要
The cold shock domain (CSD) is the most conserved nucleic acid binding domain and is distributed from bacteria to animals and plants. CSD proteins are RNA chaperones that destabilize RNA secondary structures to regulate stress tolerance and development. AtCSP2 is one of the four CSD proteins in Arabidopsis and is up-regulated in response to cold. Since AtCSP2 negatively regulates freezing tolerance, it was proposed to be a modulator of freezing tolerance during cold acclimation. Here, we examined the function of AtCSP2 in seed germination. We found that AtCSP2-overexpressing lines demonstrated retarded germination as compared with the wild type, with or without stress treatments. The ABA levels in AtCSP2-overexpressing seeds were higher than those in the wild type. In addition, overexpression of AtCSP2 reduced the expression of an ABA catabolic gene (CYP707A2) and gibberellin biosynthesis genes (GA20ox and GA3ox). These results suggest that AtCSP2 negatively regulates seed germination by controlling ABA and GA levels.
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