鼠李糖乳杆菌
转录组
益生元
生物
益生菌
乳铁蛋白
拉伤
代谢途径
基因
乳酸菌
新陈代谢
生物化学
微生物学
细菌
基因表达
遗传学
解剖
作者
Zhen-Shu Liu,Chuen‐Fu Lin,Po‐Wen Chen
出处
期刊:Journal of Food and Drug Analysis
[The Journal of Food and Drug Analysis (JFDA), Food and Drug Administration, Taiwan (TFDA)]
日期:2021-09-13
卷期号:29 (3): 402-418
被引量:6
标识
DOI:10.38212/2224-6614.3369
摘要
Lactoferrin (LF) is secreted by mammals and displays extensive biological effects. We previously reported that bovine LF (BLF) can boost the cold tolerance of a well-applied probiotic strain, Lactobacillus rhamnosus GG (LGG), to grow robustly under a cold environment, but the molecular mechanism is not clear. Here, RNA-seq analysis was conducted to ascertain molecular pathways underlying cold tolerance exerted by BLF. LGG was cultured in a cold environment (22°C) in the presence or absence of BLF. Transcriptome analysis indicated that BLF significantly elicited 1.2 to 3.2 fold (log2 Fold change) higher expression levels of genes related to stress, defense, cell division, and transporter in LGG, including the genes CspA, LytR, XRE, MerR, and GpsB. The KEGG pathway and GO analyses confirmed that BLF can modulate a few central pathways to boost the growth of LGG. BLF also reduced metabolic pathways involved in purine, amino acid, pyrimidine, one-carbon metabolism, and secondary metabolites in LGG. We speculate that the reduction of the above pathways may play key roles to reduce energy requirement and maintain carbon metabolism balance in LGG for surviving and growing in a cold state, and BLF can be an excellent prebiotic to LGG cultured in this cold condition (22°C). Overall, this study uncovers the molecular effects of BLF on LGG.
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