Anti‐gastric cancer activity and mechanism of natural compound “Heilaohulignan C” isolated from Kadsura coccinea

In this research, we analyzed the antitumor activity of one new compound Heilaohulignan C (B‐6) on the human gastric carcinoma cells. MTT, cell migration, Calcein AM/Propidium Iodide (PI), and flow cytometry in BGC‐823 cell line (gastric tumor). Western blot was utilized to distinguish the protein level. Xenografts nude mice were used for in vivo anticancer analysis. H&E staining and laboratory investigation was accomplished for toxicity study. MTT test demonstrated the cytotoxicity of BGC‐823 cells, Calcein AM/Propidium Iodide (PI) examine indicated increment dead cells proportion with a high dose of B‐6, Flow cytometry (FACS) measure showed that B‐6 influenced gastric cancer cells by initiating apoptosis. Western blot analysis confirmed that (B‐6) decrease the level of Bcl‐2 and increase the level of p53, Bax, and cleaved Caspase‐3, this confirms that the B‐6 doing the apoptosis through caspase and cytochrome C apoptotic pathways. Also, B‐6 particularly decline the tumor volume and tumor size in the xenograft mice. H&E staining additionally supports that B‐6 does not have any toxic impact on the normal tissues. This research supports that B‐6 have pharmacological activity against gastric cancer, by p53 and mitochondrial dependent apoptotic pathway, and have no toxicity on normal tissues.