Automated morphometric endothelial analysis combined with video specular microscopy.

ABSTRACT Contact and noncontact specular microscopes were tested in combination with a highly sensitive video residual-light camera and with the Kontron IPS (Image Processing System). The video information is recorded with a video recorder or with the IPS frame grabber, a digital image storage system. Examples of experimental and clinical applications are demonstrated. Nine linear and structural parameters are analyzed. Two endothelium-typical parameters are presented, which allow topological as well as hexagonal-specific geometric endothelial single-cell analysis.