Determination of the phytochemical screening, total polyphenols, flavonoids content, and antioxidant activity of soursop leaves (Annona muricata Linn.)

Abstract Annona muricata Linn. (soursop) plant is a medicinal plant that has been utilized in folk medicine for treatment of infectious and inflammatory diseases. This study aimed to determine the phytochemicals content, total phenolic and flavonoid contents and antioxidant activities of A. muricata leaves. Antioxidant activity was performed via 2, 2’-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-diphenyl- 1-picrylhydrazyl (DPPH) radical scavenging assays. The phytochemical was extracted separately with distilled water and 96% ethanol by maceration. The results show that a wide variety of pharmacologically active compounds such as alkaloids, coumarins, tannins, flavonoids, carbohydrates, phenols, terpenoids, saponins were present in the leaves of A. muricata. The total phenolic and flavonoid content of ethanol extract in A. muricata leave achieved 609.08±5.82 mgGAE/g and 209.52±1.88 mgQE/g, respectively. The antioxidant activity of the ethanol extract of A. muricata was correlated with total phenolic and flavonoid content with values IC 50 of 20.75±0.28 μg/ml, 12.84±0.21 μg/ml for DPPH and ABTS scavenging activity, respectively.