再生(生物学)
伤口愈合
组织工程
生物
生物医学工程
去细胞化
软骨
干细胞
基质(化学分析)
脚手架
软骨发生
作者
Bin Yao,Rui Wang,Yihui Wang,Yijie Zhang,Tian Hu,Wei Song,Zhao Li,Sha Huang,Xiaobing Fu
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2020-03-01
卷期号:6 (10)
被引量:24
标识
DOI:10.1126/sciadv.aaz1094
摘要
Mesenchymal stem cells (MSCs) encapsulation by three-dimensionally (3D) printed matrices were believed to provide a biomimetic microenvironment to drive differentiation into tissue-specific progeny, which made them a great therapeutic potential for regenerative medicine. Despite this potential, the underlying mechanisms of controlling cell fate in 3D microenvironments remained relatively unexplored. Here, we bioprinted a sweat gland (SG)-like matrix to direct the conversion of MSC into functional SGs and facilitated SGs recovery in mice. By extracellular matrix differential protein expression analysis, we identified that CTHRC1 was a critical biochemical regulator for SG specification. Our findings showed that Hmox1 could respond to the 3D structure activation and also be involved in MSC differentiation. Using inhibition and activation assay, CTHRC1 and Hmox1 synergistically boosted SG gene expression profile. Together, these findings indicated that biochemical and structural cues served as two critical impacts of 3D-printed matrix on MSC fate decision into the glandular lineage and functional SG recovery.
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