细菌人工染色体
转基因
转基因小鼠
计算生物学
构造(python库)
生物
同源重组
功能(生物学)
基因
遗传学
计算机科学
基因组
程序设计语言
作者
X. William Yang,Shiaoching Gong
标识
DOI:10.1002/0471142301.ns0520s31
摘要
Abstract This unit provides a comprehensive overview on the generation of transgenic mice using bacterial artificial chromosomes (BACs), and the application of BAC transgenic mice in neuroscience research. In the first section, advantages of the BAC transgenic approach compared to the conventional transgenic approach are summarized. In the second section, important considerations in designing BAC transgenic constructs are outlined. Four commonly used BAC transgenic construct designs are also outlined. Concepts of modifying BACs by homologous recombination in E. coli to introduce a variety of mutations into BACs, and important steps to characterize a modified BAC prior to the generation of transgenic mice are also presented. In the final section, some of the important applications of BAC transgenic mice in neuroscience research, including studying gene expression, gene function, mapping neuronal circuitry, and modeling human diseases, are described.
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