The biosynthesis of crustacean chitin by a microsomal enzyme from larval brine shrimp.

MaterialsHepes,' N-acetylglucosamine, chitinase, chitosan, dithiothreitol, DEAE-Sephacel, and crude chitin were purchased from Sigma.['HIAcetic anhydride (399 mCi/mmol), UDP-N-acetyl-~-[6-'HH]glucosamine (6.6 Ci/mmol), and UDP-N-acety~-~-[l-'~C]glucosamine (50 mCi/mmol) were the products of New England Nuclear.UDP